7CM8
High resolution crystal structure of M92A mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-07-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.440, 112.440, 44.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.560 - 1.900 |
| R-factor | 0.20087 |
| Rwork | 0.199 |
| R-free | 0.23076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xcp |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.145 | 0.820 |
| Number of reflections | 21690 | 1380 |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 98.9 | 98.6 |
| Redundancy | 10.2 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 100mM HEPES buffer pH 7.4, 1.4M Sodium Citrate |
