7CM2
The Crystal Structure of human USP7 USP domain from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.050, 69.131, 76.858 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 19.685 - 2.250 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.685 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.066 | 0.660 |
| Number of reflections | 37704 | 3477 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH8.5, 25% PEG3,350 |
