7CM1

Neuraminidase from the Wuhan Asiatic toad influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	119.966, 143.148, 120.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.350 - 2.140
R-factor	0.2234
Rwork	0.220
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9s
RMSD bond length	0.007
RMSD bond angle	0.994
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3940)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.140	2.140
Number of reflections	55993	5151
<I/σ(I)>	7.2
Completeness [%]	97.6
Redundancy	3.5
CC(1/2)	0.995	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	8 mg/mL in 20 mM Tris, 50 mM NaCl pH 8.0