7CLK
Mycobacterium tuberculosis enolase in complex with alternate 2-phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-03-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 139.804, 139.804, 89.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.990 - 2.150 |
| R-factor | 0.1448 |
| Rwork | 0.142 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j04 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.545 |
| Number of reflections | 29616 | 2875 |
| <I/σ(I)> | 13.39 | |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2M ammonium acetate, Bis-Tris pH 6.5 |
