7CLG
Crystal structure of the ATP-dependent restriction endonuclease SauUSI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.583, 175.679, 89.379 |
| Unit cell angles | 90.00, 115.68, 90.00 |
Refinement procedure
| Resolution | 56.790 - 3.100 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.29670 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.752 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.800 | 3.220 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | 1.200 |
| Number of reflections | 85717 | 4572 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium citrate, 11% to 14% PEG 4000' 0.25 M to 0.4 M ammonium sulfate |
