7CKV
Crystal structure of Cyanobacteriochrome GAF domain in Pr state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2020-01-29 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.730, 101.334, 41.810 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 38.663 - 1.630 |
| Rwork | 0.158 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vv4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.718 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.790 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.046 | 0.483 |
| Rmeas | 0.054 | 0.562 |
| Rpim | 0.027 | 0.285 |
| Number of reflections | 36876 | 1810 |
| <I/σ(I)> | 13.3 | 2.2 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.996 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 27% PEG 4000, 0.2M MgCl2, 0.1M Tris-HCl |
