7CJ3

Crystal structure of the transmembrane domain of Salpingoeca rosetta rhodopsin phosphodiesterase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.550, 74.140, 117.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.110 - 2.600
R-factor	0.24852
Rwork	0.246
R-free	0.29461
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0m
RMSD bond length	0.009
RMSD bond angle	1.673
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.160	2.760
High resolution limit [Å]	2.600	2.600
Rmeas	0.845	1317.700
Number of reflections	18193	2909
<I/σ(I)>	7.13
Completeness [%]	99.9
Redundancy	62.4
CC(1/2)	0.996	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	29-35% (w/v) PEG400, 100 mM Na-citrate, pH 5.0, 100 mM KSCN and 10 mM ZnCl2