7CJ0

Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	47.510, 47.510, 616.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.080 - 2.500
R-factor	0.2607
Rwork	0.258
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bnv
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.084	41.080	2.300
High resolution limit [Å]	2.240	10.020	2.240
Rmerge	0.086	0.043	2.034
Number of reflections	41413	621	2958
<I/σ(I)>	17.7
Completeness [%]	99.9	99	99.1
Redundancy	17.3	13.7	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.17 M Sodium acetate, 0.1 M Tris, pH8.5, 25% (v/v) PEG 4000, 20% (v/v) Glycerol