7CHS

Crystal structure of SARS-CoV-2 antibody P22A-1D1 with RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9769
Spacegroup name	C 1 2 1
Unit cell lengths	193.337, 86.598, 57.160
Unit cell angles	90.00, 99.25, 90.00

Refinement procedure

Resolution	47.706 - 2.401
R-factor	0.1925
Rwork	0.191
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.114	0.983
Number of reflections	36392	3573
<I/σ(I)>	13.1
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.989	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M potassium chloride, 0.1M NaHEPES, pH 7.0, 15% PEG 5000MME