7CHD

AtaT complexed with acetyl-methionyl-tRNAfMet

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	269.830, 68.280, 136.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.214 - 3.804
R-factor	0.2892
Rwork	0.286
R-free	0.34090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gtp
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.214	48.214	3.900
High resolution limit [Å]	3.800	17.010	3.800
Rmerge	0.441	0.083	3.416
Rmeas	0.449	0.086	3.483
Total number of observations	668160
Number of reflections	25540	323	1813
<I/σ(I)>	8.36	31.78	1.25
Completeness [%]	99.8	94.4	99
Redundancy	26.161	18.254	26.185
CC(1/2)	0.999	1.000	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Hepes 6.8, 0.2M Sodium formate, 10% PEG3350, 0.5% Jeffemine