7CHB

Crystal structure of the SARS-CoV-2 RBD in complex with BD-236 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	195.614, 86.788, 56.415
Unit cell angles	90.00, 100.13, 90.00

Refinement procedure

Resolution	48.140 - 2.400
R-factor	0.194
Rwork	0.192
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w41
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.18_3845)
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	36363	3591
<I/σ(I)>	15.2
Completeness [%]	99.6
Redundancy	6.8
CC(1/2)	0.976	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium Citrate, pH 5.0, 8% (w/v) PEG 8000