7CG5

Structure of the sensor domain (long construct) of the anti-sigma factor RsgI4 in Pseudobacteroides cellulosolvens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-25
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97849
Spacegroup name	H 3 2
Unit cell lengths	71.230, 71.230, 127.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.610 - 2.850
R-factor	0.2449
Rwork	0.241
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cg1
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	XDS (20191211)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	2.920
High resolution limit [Å]	2.850	2.850
Rmeas	0.174	0.672
Number of reflections	5638	401
<I/σ(I)>	9.12	2.58
Completeness [%]	100.0	100
Redundancy	10.1	10.2
CC(1/2)	0.995	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	291	0.1 M phosphate-citrate (pH4.2), 0.2 M lithium sulfate and 19% PEG1000