7CFF

Structure of the thermostabilized transmembrane domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 2 2 2
Unit cell lengths	57.490, 83.320, 98.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 2.000
R-factor	0.2171
Rwork	0.216
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.120
High resolution limit [Å]	2.000	2.000
Number of reflections	15683	2447
<I/σ(I)>	6.99
Completeness [%]	95.8
Redundancy	4.7
CC(1/2)	0.983	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4	291	10 mM ZnCl2, 100 mM sodium acetate (pH 4.0), 40% polyethylene glycol 200