7CFD

Drosophila melanogaster Krimper eTud2-AubR15me2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	97.118, 101.365, 191.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.992 - 2.704
R-factor	0.2453
Rwork	0.243
R-free	0.29040
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.114	0.511
Rmeas	0.127	0.588
Rpim	0.055	0.284
Number of reflections	50060	3860
<I/σ(I)>	12.5	1.52
Completeness [%]	95.5	75.1
Redundancy	5.1	3.7
CC(1/2)		0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	16% PEG3350 and 0.1 M Ammonium acetate