7CFC

Drosophila melanogaster Krimper eTud1-Ago3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 31
Unit cell lengths	88.464, 88.464, 181.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.513 - 2.400
R-factor	0.2354
Rwork	0.234
R-free	0.25890
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.094	0.891
Rmeas	0.107
Rpim	0.050	0.467
Number of reflections	61822	6092
<I/σ(I)>	9	1.25
Completeness [%]	99.3	98.6
Redundancy	4.3	4.3
CC(1/2)	0.992	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES pH 7.5, 1.5M lithium sulfate