7CFA
Crystal structure of the restriction DNA glycosylase R.CcoLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-11-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.493, 89.596, 100.658 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.928 - 2.355 |
| R-factor | 0.2306 |
| Rwork | 0.229 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iff |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.384 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.170 | 47.170 | 2.440 |
| High resolution limit [Å] | 2.350 | 8.810 | 2.350 |
| Rmerge | 0.050 | 0.023 | 0.865 |
| Rmeas | 0.054 | 0.025 | 0.940 |
| Rpim | 0.020 | 0.010 | 0.364 |
| Total number of observations | 183674 | 3304 | 17258 |
| Number of reflections | 25594 | 560 | 2631 |
| <I/σ(I)> | 23.1 | 69.1 | 2.1 |
| Completeness [%] | 99.9 | 98.5 | 99.7 |
| Redundancy | 7.2 | 5.9 | 6.6 |
| CC(1/2) | 0.999 | 0.999 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0 and 8% PEG6000 |
