7CE5

Methanol-PQQ bound methanol dehydrogenase (MDH) from Methylococcus capsulatus (Bath)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	128.612, 211.828, 223.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.000 - 1.800
Rwork	0.140
R-free	0.17000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tqo
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	535098	176635
<I/σ(I)>	14.11
Completeness [%]	99.8
Redundancy	3.5
CC(1/2)	0.900	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291.15	Hepes, PEG600