7CE1
Complex STRUCTURE OF TRANSCRIPTION FACTOR SghR with its COGNATE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.900, 218.960, 284.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.149 - 3.200 |
| R-factor | 0.2358 |
| Rwork | 0.233 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cdv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 987667 | 111573 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10mM Tris-HCl pH 8.5, 100mM NaCl, 1mM EDTA |
