7CDB

Structure of GABARAPL1 in complex with GABA(A) receptor gamma 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97872
Spacegroup name	P 61 2 2
Unit cell lengths	89.379, 89.379, 115.004
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.681 - 1.949
R-factor	0.1991
Rwork	0.197
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r2q
RMSD bond length	0.008
RMSD bond angle	0.967
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.949	5.290	1.950
Rmerge	0.220	0.076	0.961
Rmeas	0.228	0.079	0.999
Rpim	0.059	0.020	0.265
Number of reflections	19730	1069	967
<I/σ(I)>	4.3
Completeness [%]	96.4	90.7	98.3
Redundancy	14.3	13.8	13.9
CC(1/2)		0.998	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	289	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dehydrate pH 5.6, and 30% PEG 4000