7CCH
Acinetobacter baumannii histidine kinase AdeS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 141.052, 141.052, 50.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.718 - 2.848 |
R-factor | 0.2523 |
Rwork | 0.249 |
R-free | 0.28380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lfk |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.718 | 2.950 |
High resolution limit [Å] | 2.848 | 2.850 |
Rmeas | 0.071 | |
Number of reflections | 7192 | 708 |
<I/σ(I)> | 35.5 | |
Completeness [%] | 97.5 | |
Redundancy | 25.3 | |
CC(1/2) | 1.000 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.7 M Sodium citrate tribasic dehydrate, 0.1 M BIS-TRIS propane pH 7.0 |