7CCB
Crystal structure of the SPRY domain-containing protein 7 (SPRY7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.060, 75.910, 81.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.610 - 1.620 |
R-factor | 0.1637 |
Rwork | 0.162 |
R-free | 0.19020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ji7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.753 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.160 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.122 | 0.634 |
Number of reflections | 44152 | 2222 |
<I/σ(I)> | 11.7 | 3.8 |
Completeness [%] | 100.0 | |
Redundancy | 8.5 | |
CC(1/2) | 0.996 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2% v/v Tacsimate pH 6.0, 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 3,350 |