7CC7

Tetratricopeptide repeat (TPR) domain of protein tyrosine phosphatase-interacting protein 51 (PTPIP51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97903
Spacegroup name	P 61 2 2
Unit cell lengths	80.025, 80.025, 202.893
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.250 - 1.450
R-factor	0.1928
Rwork	0.192
R-free	0.21310
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	2.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.075	0.771
Rmeas	0.079	0.851
Rpim	0.024	0.353
Number of reflections	68214	4398
<I/σ(I)>	58.5	3.27
Completeness [%]	99.0	98.3
Redundancy	10.3	5.5
CC(1/2)		0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM Tris-HCl pH 8.0 and 30% (w/v) SOKALAN CP-42