7CAV

Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: co-FG+DMAPP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	98.853, 98.910, 193.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 1.910
Rwork	0.196
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7caq
RMSD bond length	0.005
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	1.940
High resolution limit [Å]	1.910	1.910
Rmerge		2.050
Number of reflections	147512	7217
<I/σ(I)>	33.6
Completeness [%]	100.0
Redundancy	22.3
CC(1/2)	0.688	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.075 M succinic acid, pH7.0, 11% PEG 3350, and 25% glycerol, 3 mM farnesyl pyrophosphate (FPP), 0.3 mM MgCl2