7CAP
Cyclic Lys48-linked triubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-06-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.429, 54.820, 60.954 |
| Unit cell angles | 90.00, 121.99, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.330 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.742 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.075 | 0.784 |
| Number of reflections | 56837 | 2796 |
| <I/σ(I)> | 25.2 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 20% PEG 3350, 200 mM zinc acetate |
