7C91
Blasnase-T13A with D-asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.260, 92.260, 231.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.246 - 1.980 |
| Rwork | 0.182 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c8x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.246 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rpim | 0.031 | 0.221 |
| Number of reflections | 70317 | 5096 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 25.7 | |
| CC(1/2) | 0.997 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | Formate |
