7C68

Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-07-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.630, 109.740, 111.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.930 - 2.050
R-factor	0.184
Rwork	0.182
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.013
RMSD bond angle	1.828
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.780	55.780	2.110
High resolution limit [Å]	2.050	8.940	2.050
Rmerge	0.131	0.067	0.505
Rmeas	0.142	0.073	0.545
Rpim	0.052	0.028	0.201
Total number of observations	352325	4715	27050
Number of reflections	48888	692	3760
<I/σ(I)>	9.6	17.6	3.2
Completeness [%]	99.7	99.8	99.4
Redundancy	7.2	6.8	7.2
CC(1/2)	0.993	0.995	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulphate, 40% PEG 8000