7C67

Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-07-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.670, 109.880, 110.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.000 - 2.000
R-factor	0.1727
Rwork	0.170
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.013
RMSD bond angle	1.766
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.670	62.670	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.107	0.055	0.529
Rmeas	0.115	0.060	0.572
Rpim	0.042	0.023	0.212
Total number of observations	368548	4758	25412
Number of reflections	52166	691	3744
<I/σ(I)>	9.6	18	3.1
Completeness [%]	99.4	99.7	98.3
Redundancy	7.1	6.9	6.8
CC(1/2)	0.996	0.996	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulphate, 40% PEG 8000