7C63

Crystal structure of beta-glycosides-binding protein of ABC transporter in an open state (Form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.520, 62.930, 104.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.000 - 1.630
R-factor	0.1661
Rwork	0.164
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uor
RMSD bond length	0.017
RMSD bond angle	2.163
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.83)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.000	51.000	1.660
High resolution limit [Å]	1.630	8.920	1.630
Rmerge	0.049	0.020	0.309
Rmeas	0.052	0.022	0.330
Rpim	0.017	0.008	0.115
Total number of observations	440139	2818	19653
Number of reflections	48845	363	2397
<I/σ(I)>	24.7	52.6	4.9
Completeness [%]	100.0	99.5	99.8
Redundancy	9	7.8	8.2
CC(1/2)	0.999	1.000	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulphate, 30% PEG 8000