7C5Z

Crystal structure of spring viremia of carp virus phosphoprotein central domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	293
Detector technology	CCD
Collection date	2018-01-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	37.900, 54.110, 89.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.920 - 1.500
R-factor	0.2079
Rwork	0.207
R-free	0.22560
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.799
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.930	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	30365	2184
<I/σ(I)>	13.7
Completeness [%]	99.9	100
Redundancy	12.7
CC(1/2)	0.998	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289.15	0.1 M Ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% PEG 10000