7C5P
Crystal Structure Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with G3P at 2.35 Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.339, 90.339, 341.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.15778 |
| Rwork | 0.155 |
| R-free | 0.20975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.793 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.190 | 0.624 |
| Number of reflections | 60156 | 5632 |
| <I/σ(I)> | 14 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 100mM sodium PBS pH 6.1, 16%(w/v) PEG 1000, 200mM MgCl2 |
