7C4M
Ancestral L-amino acid oxidase (AncLAAO-N5) L-Trp binding form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 132.499, 132.499, 191.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.930 - 2.400 |
| R-factor | 0.2112 |
| Rwork | 0.208 |
| R-free | 0.26960 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.930 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.107 | 0.596 |
| Number of reflections | 33554 | 4819 |
| <I/σ(I)> | 30.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 26.8 | |
| CC(1/2) | 1.000 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295 | 1.0M NaCl, 0.1M citric acid pH 3.5 |
