7C4H
Crystal structure of BCP1 from Saccharomyces Cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97622 |
Spacegroup name | P 21 3 |
Unit cell lengths | 127.794, 127.794, 127.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.090 - 1.830 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.19490 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.083 | 0.423 |
Number of reflections | 61457 | 6068 |
<I/σ(I)> | 26.17 | 6.03 |
Completeness [%] | 100.0 | |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 283 | 100 mM sodium citrate tribasic dihydrate (pH 5.2), 32% v/v polyethylene glycol 200 |