7C47

The crystal structure of Trypanosoma brucei RNase D : CMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	52.333, 77.350, 102.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.2229
Rwork	0.221
R-free	0.25000
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.058	0.029	0.369
Rmeas	0.061	0.031	0.398
Rpim	0.019	0.009	0.143
Total number of observations	203951
Number of reflections	21563	2340	1990
<I/σ(I)>	8.6
Completeness [%]	98.6	99.7	91.5
Redundancy	9.5	11.6	6.4
CC(1/2)		0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 0% PEG 3350