7C42
The crystal structure of Trypanosoma brucei RNase D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-04-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.929, 77.451, 101.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.497 - 2.000 |
R-factor | 0.1793 |
Rwork | 0.176 |
R-free | 0.21520 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
High resolution limit [Å] | 1.998 | 4.310 | 2.000 |
Rmerge | 0.116 | 0.100 | 0.622 |
Rmeas | 0.121 | 0.105 | 0.660 |
Rpim | 0.036 | 0.030 | 0.214 |
Number of reflections | 42575 | 2974 | 2524 |
<I/σ(I)> | 9.6 | ||
Completeness [%] | 98.8 | 99.9 | 92.5 |
Redundancy | 10.2 | 11.9 | 7.3 |
CC(1/2) | 0.992 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1 M Bis-Tris pH 5.5, 30% PEG 3350 |