7C2G
Crystal Structure of the Thorarchaeota 2DGel/rabbit actin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.854, 101.343, 55.765 |
Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
Resolution | 19.862 - 1.710 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.20270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hbt |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.600 | 1.700 |
Rmerge | 0.100 | 0.088 | 0.914 |
Rmeas | 0.108 | 0.095 | 1.026 |
Rpim | 0.042 | 0.036 | 0.455 |
Total number of observations | 403297 | ||
Number of reflections | 60879 | 3166 | 2893 |
<I/σ(I)> | 12 | ||
Completeness [%] | 98.0 | 99.9 | 92.5 |
Redundancy | 6.6 | 6.9 | 4.5 |
CC(1/2) | 0.985 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 0.1 M Bis-Tris pH 5.5 0.2 M magnesium chloride hexahydrate 25% w/v polyethylene glycol 3350 |