7C2F
Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.427, 108.626, 167.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.590 - 2.030 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hbt |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.140 | 2.080 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.115 | 0.790 |
Rmeas | 0.125 | 0.856 |
Rpim | 0.048 | 0.327 |
Number of reflections | 68445 | 4283 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.5 | 93 |
Redundancy | 6.7 | 6.6 |
CC(1/2) | 0.998 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 0.1 M MES pH 6.0 0.2 M magnesium chloride hexahydrate 20% w/v polyethylene glycol 6000 |