7C2B

Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin f2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	68.738, 83.943, 69.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.730 - 1.795
R-factor	0.1809
Rwork	0.179
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pu9
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	1.859
High resolution limit [Å]	1.795	1.795
Rmerge	0.104	0.992
Rmeas	0.113	1.070
Rpim	0.043	0.398
Number of reflections	37811	3690
<I/σ(I)>	13.48	2.3
Completeness [%]	99.4	98.48
Redundancy	6.8	7
CC(1/2)	0.998	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	200 mM Mg Nitrate 16-20 % PEG 3,350