7C25
Glycosidase Wild Type at pH8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 105.462, 105.462, 89.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.209 - 1.505 |
| Rwork | 0.136 |
| R-free | 0.17260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z3a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.210 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.077 | 1.357 |
| Number of reflections | 74640 | 12800 |
| <I/σ(I)> | 0.265 | 0.028 |
| Completeness [%] | 92.6 | 99.7 |
| Redundancy | 26.1 | 24.2 |
| CC(1/2) | 1.000 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium citrate (pH8.0), 8% PEG monomethyl ether 2000 |
