7C24
Glycosidase F290Y at pH8.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 104.806, 104.806, 89.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.590 - 1.710 |
Rwork | 0.163 |
R-free | 0.18940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z3d |
RMSD bond length | 0.010 |
RMSD bond angle | 1.650 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.590 | 1.810 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmeas | 0.226 | 4.667 |
Number of reflections | 54864 | 8723 |
<I/σ(I)> | 0.134 | 0.0084 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 26.1 | 25.5 |
CC(1/2) | 0.999 | 0.529 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium citrate (pH 8.0), 8% PEG monomethyl ether 2000 |