7C22

Crystal structure of the C-terminal domain of SARS-CoV-2 nucleocapsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 1
Unit cell lengths	43.763, 49.458, 68.819
Unit cell angles	106.79, 90.05, 97.79

Refinement procedure

Resolution	19.820 - 2.000
R-factor	0.191
Rwork	0.189
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjr
RMSD bond length	0.007
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.107	0.639
Rpim	0.065	0.391
Number of reflections	35328	2636
<I/σ(I)>	5.9	1.4
Completeness [%]	95.6	96
Redundancy	3.5
CC(1/2)	0.995	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Ammonium acetate, 0.1M Sodium acetate pH 4.6, 30% PEG 4000