7C1X
Unliganded structure of Pseudouridine kinase (PUKI) from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.686, 48.352, 92.751 |
Unit cell angles | 90.00, 109.14, 90.00 |
Refinement procedure
Resolution | 29.932 - 2.389 |
R-factor | 0.210811925093 |
Rwork | 0.208 |
R-free | 0.24225 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.893 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.380 | 2.400 |
Number of reflections | 26768 | 781 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 99.2 | |
Redundancy | 3.4 | |
CC(1/2) | 0.973 | 0.728 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M tri sodium citrate, 22%PEG 3350 |