7C1K
Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant R148A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-19 |
| Detector | SIEMENS-NICOLET |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.965, 89.701, 98.345 |
| Unit cell angles | 113.22, 90.09, 89.92 |
Refinement procedure
| Resolution | 39.121 - 2.755 |
| R-factor | 0.2385 |
| Rwork | 0.237 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c1h |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.755 | 7.590 | 2.800 |
| Rmerge | 0.067 | 0.034 | 0.366 |
| Rmeas | 0.078 | 0.040 | 0.434 |
| Rpim | 0.041 | 0.021 | 0.230 |
| Number of reflections | 45676 | 2279 | 2135 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 94.8 | 95 | 88.7 |
| Redundancy | 3.6 | 3.7 | 3.4 |
| CC(1/2) | 0.997 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 289 | Citric acid, BIS-TRIS propane, PEG 3350 |
