7C0N

Crystal structure of a self-assembling galactosylated peptide homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	293
Detector technology	CCD
Collection date	2019-06-08
Detector	RAYONIX MX-300
Wavelength(s)	1.54191
Spacegroup name	F 41 3 2
Unit cell lengths	72.837, 72.837, 72.837
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.752 - 1.552
Rwork	0.234
R-free	0.24950
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.078	0.369
Number of reflections	2694	256
<I/σ(I)>	54.2	17
Completeness [%]	100.0
Redundancy	72.6	63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	40%(w/v) PEG 1000, 0.1 M Sodium phosphate dibasic/Citric acid(pH 4.2), 0.2 M Lithium sulfate