7C0G

Aca1 in complex with 14bp palindromic DNA target

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97890
Spacegroup name	P 61 2 2
Unit cell lengths	68.727, 68.727, 206.006
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.400
R-factor	0.2201
Rwork	0.217
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7C0B
RMSD bond length	0.011
RMSD bond angle	1.413
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (phenix-1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.133	0.814
Number of reflections	12045	1108
<I/σ(I)>	24.333
Completeness [%]	99.5
Redundancy	27.4
CC(1/2)	1.000	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Sodium acetate pH5.0; 20%(w/v) MPD