7C0F
Crystal structure of a dinucleotide-binding protein of ABC transporter endogenously bound to uridylyl-3'-5'-phospho-guanosine (Form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-03-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.160, 120.880, 66.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.060 - 2.150 |
R-factor | 0.1604 |
Rwork | 0.158 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mfi |
RMSD bond length | 0.015 |
RMSD bond angle | 1.927 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (7.2.2) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.060 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.105 | 0.429 |
Rmeas | 0.148 | 0.594 |
Rpim | 0.104 | 0.410 |
Number of reflections | 48571 | 3803 |
<I/σ(I)> | 6.9 | 2.5 |
Completeness [%] | 98.2 | 95.1 |
Redundancy | 2.8 | 2.7 |
CC(1/2) | 0.987 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M ammonium phosphate, 0.1M sodium cacodylate pH 6.5, 30% PEG 8000 |