7C07
Crystal structure of yeast U2AF1 complex bound to 5'-AAGGU RNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.487, 256.947, 94.591 |
Unit cell angles | 90.00, 100.75, 90.00 |
Refinement procedure
Resolution | 48.170 - 3.200 |
R-factor | 0.2563 |
Rwork | 0.255 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yh8 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.447 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.170 | 3.270 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.117 | 0.802 |
Number of reflections | 72802 | 4522 |
<I/σ(I)> | 10.2 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.9 | 4.8 |
CC(1/2) | 0.996 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M NaCitrate, 10% PEG 35000 |