7C03

Crystal structure of POLArISact(T57S), genetically encoded probe for fluorescent polarization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	I 21 21 21
Unit cell lengths	78.130, 113.590, 132.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.202 - 2.501
R-factor	0.2165
Rwork	0.214
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3evp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	20785	45440
<I/σ(I)>	13.3
Completeness [%]	99.9
Redundancy	7.1
CC(1/2)	0.912	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate (pH4.5-5.0), 6-8% (v/v) PEG 4000