7C00

Crystal structure of the SRCR domain of human SCARA5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	38.167, 48.802, 70.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.647 - 1.700
R-factor	0.1597
Rwork	0.158
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SRCR domain of mouse SCARA5
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.650	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.082	0.393
Number of reflections	16428	1606
<I/σ(I)>	29.03
Completeness [%]	99.6
Redundancy	12.7	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	40% (w/v) polyethylene glycol 400, 0.2M sodium chloride, 0.1M Tacsimate (pH 8.0)