7BZM

Crystal structure of rice Os3BGlu7 with glucoimidazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-02
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	80.402, 101.557, 127.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.300
R-factor	0.1786
Rwork	0.177
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rgl
RMSD bond length	0.008
RMSD bond angle	1.265
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	47470	2303
<I/σ(I)>	17.3	6.6
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.998	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	288	23% PEG MME 5000, 0.17 M AMMONIUM SULFATE, 0.1M MES, PH 6.7