7BZK

Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin y1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	54.985, 60.762, 61.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.084 - 1.593
R-factor	0.171
Rwork	0.170
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pu9
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.084	1.651
High resolution limit [Å]	1.593	1.593
Rmerge	0.120	0.761
Rmeas	0.130	0.831
Rpim	0.050	0.331
Number of reflections	27925	2737
<I/σ(I)>	10.4
Completeness [%]	99.9	99.56
Redundancy	6.6	6.2
CC(1/2)	0.995	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293.15	100 mM Magnesium Citric acid pH 3.5 5% 2-propanol 5-9% PEG 3,350